To establish the in vitro transcription system for mononegaviruses

Virus researchers use in vitro transcription systems to understand the molecular mechanisms of RNA synthesis of nonsegment, single negative strand RNA viruses. We need to prepare the optimal materials including the template RNA and RNA-dependent-RNA-polymerase complex for such experiments. When we can easily collect the high amount of viral particles, we will have a good possibility to establish the system, because those particles have the complete machinery to ensure the viral transcription. Therefore, the in vitro transcription systems for vesicular stomatitis virus and Sendai virus have been established, because these RNA viruses are known to produce significant amounts of viral particle under the laboratory cell culture condition. However, in case of other RNA viruses, these systems has not been broadly available due to the lack of a suitable method to collect the enough amounts of material to perform the in vitro transcription. Accordingly, to establish the method to prepare the optimal material for in vitro transcription of such other RNA viruses is thought to be one of the interesting field of the virus study.