A method of RNA cleavage using ribonuclease H

 Ribonuclease H (RNase H) is an RNA cleavage enzyme that specifically recognize RNAs that hybridize to DNA as the substrate. By utilizing this characteristic of the enzyme, we can cleave RNAs in optimal site. At first, we hybridize a DNA oligo to the substrate RNA at the closest region of cleavage. Subsequently, we treat RNase H to the partially RNA-DNA hybridized substrate. RNase H specifically cleaves the RNA at only the DNA-hybridized area, and then we can obtain the cleaved RNA.