RNA viruses may have a proofreading function in their RNA
dependent RNA polymerases. The proofreading function exchanges an incorrectly
incorporated nucleotide into the correct one. This function was detected in
influenza virus polymerase by cell free transcription system for example.
Influenza virus polymerase makes GTP residues after the first nucleotide after
the primer for initiation of transcription in the presence of the high concentrations of GTP. This GTP
incorporated position should be actually set for CTP. However, the second nucleotide
was removed by viral polymerase, and exchanged into CTP when the optimal amount
of CTP exists. This experiment suggests the presence of a proofreading function
in influenza virus polymerase.
I want to demonstrate whether other RNA viruses have
same function in their RNA synthesis machinery. It is very important to understand the fidelity of RNA virus
polymerases. RNA viruses are known as highly mutation-prone pathogens in their
genome, indicating that many mutated novel viruses appear due to the error-prone
mechanism. If a virus has a proofreading function, why does it cause many
mutations in their genome?
No comments:
Post a Comment