LIFE IS POSITIVE CONTROL
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Wednesday, April 10, 2013
Molecular biology protocol: recipes of 2M DTT and 0.2M Spermidine
2M Dithiothreitol (DTT):
reducing agent
DTT 3.09g
0.01M Sodium acetate (pH 5.2) 10ml
Sterilized by filtration
Store at -20℃
0.2M Spermidine:
T7 polymerase activator
Spermidine 29mg
H
2
O 1ml
Sterilized by filtration
Store at -20℃
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