An expression library of 407 human interferon-stimulated genes (ISGs) that were individually inserted in a plasmid was co-transfected in culture cells together with a modified Ebola virus (EBOV) minigenome system expressed as virus-like particles. Several genes that influenced the EBOV reporter activity were identified, and E3 Ubiquitin-Ligase TRIM25 was shown to be an antiviral factor against EBOV replication. TRIM25 was found to be interact with viral proteins associated with incoming EBOV genome. TRIM25 promotes the ubiquitination of viral nucleoprotein (NP), and is involved in the dissociation of NP from viral RNA.
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