The spike gene-deleted SARS-CoV-2 self-replicating RNA replicon was developed by using a yeast-based reverse genetics system. This non-infectious replicon can be trans-complemented by expressing glycoprotein of SARS-CoV-2 or vesicular stomatitis virus to generate replicon particle for single-cycle delivery into a range of cell types. This system is useful for studying antiviral drug screening, neutralization assay, host factor validation and characterizing viral variants.
No comments:
Post a Comment